E-cadherin antibody

Catalog No. ABIN2854751

Product Details anti-E-cadherin Antibody

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Whole Mount) (IHC (wm))

Cross-Reactivity: Dog, Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to E-Cadherin (cadherin 1, type 1, E-cadherin (epithelial))
E-Cadherin antibody

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MCF-7

Validation: Comparison, KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.29 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-E-cadherin antibody (ABIN2854751)

Bray, Elgamal, Jiang, Wright, Sutaria, Badawi, Borcyk, Liu, Fredenburg, Campbell-Thompson, Schmittgen: "Loss of RE-1 silencing transcription factor accelerates exocrine damage from pancreatic injury." in: Cell death & disease, Vol. 11, Issue 2, pp. 138, (2020) (PubMed).

Zhang, Fang, Chen, Zhao, Wu, Pan: "Qingshen Buyang Formula Attenuates Renal Fibrosis in 5/6 Nephrectomized Rats via Inhibiting EMT and Wnt/β-Catenin Pathway." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2019, pp. 5370847, (2019) (PubMed).

Chu, Lee, Lee, Wang, Huang, Lan, Lin, Lin, Tian, Liu, Chow: "MiR-338-5p promotes metastasis of colorectal cancer by inhibition of phosphatidylinositol 3-kinase, catalytic subunit type 3-mediated autophagy pathway." in: EBioMedicine, Vol. 43, pp. 270-281, (2019) (PubMed).

Ahsan, Singh, Rocha, Huang, Prince: "Prickle1 is required for EMT and migration of zebrafish cranial neural crest." in: Developmental biology, Vol. 448, Issue 1, pp. 16-35, (2019) (PubMed).

Bacci, Aiello, Ripoli, Loria, Pugliese, Pierconti, Rotili, Strigari, Pinto, Bassi, Mai, Grassi, Pontecorvi, Falcioni, Farsetti, Nanni: "H19-Dependent Transcriptional Regulation of β3 and β4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer." in: International journal of molecular sciences, Vol. 20, Issue 16, (2019) (PubMed).

Wei, Liu, Ren, Liu, Cai, Zhu, Jin, He, Yu: "Effects of bradykinin on TGF‑β1‑induced epithelial‑mesenchymal transition in ARPE‑19 cells." in: Molecular medicine reports, Vol. 17, Issue 4, pp. 5878-5886, (2018) (PubMed).

Yang, Fu, Yao, Hou, Xue: "Runx3 is a key modulator during the epithelial-mesenchymal transition of alveolar type II cells in animal models of BPD." in: International journal of molecular medicine, Vol. 40, Issue 5, pp. 1466-1476, (2018) (PubMed).

Chamard-Jovenin, Thiebaut, Chesnel, Bresso, Morel, Smail-Tabbone, Devignes, Boukhobza, Dumond: "Low-Dose Alkylphenol Exposure Promotes Mammary Epithelium Alterations and Transgenerational Developmental Defects, But Does Not Enhance Tumorigenic Behavior of Breast Cancer Cells." in: Frontiers in endocrinology, Vol. 8, pp. 272, (2017) (PubMed).

Thiebaut, Chamard-Jovenin, Chesnel, Morel, Djermoune, Boukhobza, Dumond: "Mammary epithelial cell phenotype disruption in vitro and in vivo through ERalpha36 overexpression." in: PLoS ONE, Vol. 12, Issue 3, pp. e0173931, (2017) (PubMed).

Lee, Chen, Chen, Yang, Huang, Liu: "Reverse epithelial-mesenchymal transition contributes to the regain of drug sensitivity in tyrosine kinase inhibitor-resistant non-small cell lung cancer cells." in: PLoS ONE, Vol. 12, Issue 7, pp. e0180383, (2017) (PubMed).

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: cadherin 1 (CDH1 Products)

Background: This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.

Cellular Localization: Cell junction , Cell membrane

Molecular Weight: 97 kDa

Gene ID: 999

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation