BRCA1 antibody (C-Term)

Catalog No. ABIN2854845

This anti-BRCA1 antibody is a Rabbit Polyclonal antibody detecting BRCA1 in ELISA, IF and ICC. Suitable for Human.

Quick Overview for BRCA1 antibody (C-Term) (ABIN2854845)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-BRCA1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Dog (Canine), Rhesus Monkey, Chimpanzee

Cross-Reactivity (Details): Dog (87 %), Rhesus Monkey (100 %), Chimpanzee (100 %)

Characteristics: Rabbit Polyclonal antibody to BRCA1 (breast cancer 1, early onset)
BRCA1 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BRCA1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* ELISA-Ag Assay-dependent dilution Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* ELISA-AgAssay-dependent dilution

Comment:

Positive Control: Synthetic peptide: 1:8,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1

Background: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full-length natures identified.

Cellular Localization: Nucleus , Isoform 3: Cytoplasm , Isoform 5: Cytoplasm

Molecular Weight: 208 kDa

Gene ID: 672

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus