UCHL1 antibody (C-Term)

Catalog No. ABIN2854931

The Rabbit Polyclonal anti-UCHL1 antibody is suitable to detect UCHL1 in samples from Human. It has been validated for WB, IHC (p) and IHC (fro).

Quick Overview for UCHL1 antibody (C-Term) (ABIN2854931)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-UCHL1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to PGP9.5 (ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase))
PGP9.5 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PGP9.5. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , rat brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.56 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: ubiquitin hydrolase L1

Background: UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).[supplied by OMIM]

Cellular Localization: Cytoplasm

Molecular Weight: 25 kDa

Gene ID: 7345

UniProt: P09936

Pathways: Feeding Behaviour