Prohibitin antibody

Catalog No. ABIN2854938

This anti-Prohibitin antibody is a Rabbit Polyclonal antibody detecting Prohibitin in WB, IF, IHC (p), IP and ICC. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for Prohibitin antibody (ABIN2854938)

Target: Prohibitin (PHB)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prohibitin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-Prohibitin Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Prohibitin (prohibitin)
Prohibitin antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Prohibitin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , PC-12 , 293T

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.34 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Prohibitin antibody (ABIN2854938)

Fang, Lin, Liang, Liang: "A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and β-catenin-ABCG2 signaling." in: Journal of biomedical science, Vol. 27, Issue 1, pp. 42, (2020) (PubMed).

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: prohibitin

Background: Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.

Cellular Localization: Mitochondrion inner membrane

Molecular Weight: 30 kDa

Gene ID: 5245

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling