Factor VII antibody

Catalog No. ABIN2854983

Product Details anti-Factor VII Antibody

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Factor VII antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Upon activation of the factor VII, proteolytic cleavage of the peptide bond between Arg(152) and Ile(153) converts Factor VII (FVII) to an activated two-chain form (FVIIa). A heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated. Since the immunogen sequence locates within the heavy chain, it should recognize both Factor VII and Factor VIIa.

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit polyclonal antibody to Factor VII (coagulation factor VII (serum prothrombin conversion accelerator))
Factor VII antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Factor VII. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.32 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Factor VII

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Alternative Name: coagulation factor VII (F7 Products)

Synonyms: F7 antibody, wu:fb59f04 antibody, zgc:109870 antibody, SPCA antibody, AI114908 antibody, ATIII antibody, At-3 antibody, At3 antibody, LOC100223776 antibody, AI132620 antibody, Cf7 antibody, FVII antibody, mfVII antibody, f7 antibody, coagulation factor VII antibody, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 antibody, coagulation factor 7 (serum prothrombin conversion accelerator) S homeolog antibody, F7 antibody, f7 antibody, Serpinc1 antibody, CpipJ_CPIJ009142 antibody, CpipJ_CPIJ010295 antibody, CpipJ_CPIJ020127 antibody, fa7 antibody, f7.S antibody

Background: This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Alternative splicing of this gene results in 2 transcripts. Defects in this gene can cause coagulopathy.

Cellular Localization: Secreted

Molecular Weight: 52 kDa

Gene ID: 2155

UniProt: P08709

Pathways: Response to Growth Hormone Stimulus, Platelet-derived growth Factor Receptor Signaling