Calnexin antibody (Internal Region)

Catalog No. ABIN2855124

This anti-Calnexin antibody is a Rabbit Polyclonal antibody detecting Calnexin in WB, IF, ICC and IHC (p). Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 3+ publications.

Quick Overview for Calnexin antibody (Internal Region) (ABIN2855124)

Target: Calnexin (CANX)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Calnexin Antibody

Binding Specificity: Internal Region

Cross-Reactivity: Chicken, Dog (Canine), Xenopus laevis, Zebrafish (Danio rerio), Cow (Bovine)

Cross-Reactivity (Details): Chicken (91 %), Dog (94 %), Xenopus laevis (88 %), Zebrafish (82 %), Bovine (94 %)

Characteristics: Rabbit Polyclonal antibody to Calnexin (calnexin)
Calnexin antibody [N3C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 , PC-12 , Rat2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.87 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Calnexin antibody (ABIN2855124)

de Jong, Murphy, Mäger, Willms, Garcia-Guerra, Gitz-Francois, Lefferts, Gupta, Steenbeek, van Rheenen, El Andaloussi, Schiffelers, Wood, Vader: "A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA." in: Nature communications, Vol. 11, Issue 1, pp. 1113, (2020) (PubMed).

Ilahibaks, Lei, Mol, Deshantri, Jiang, Schiffelers, Vader, Sluijter: "Biofabrication of Cell-Derived Nanovesicles: A Potential Alternative to Extracellular Vesicles for Regenerative Medicine." in: Cells, Vol. 8, Issue 12, (2020) (PubMed).

Sever, Vo, Bols, Dixon: "Rainbow trout (Oncorhynchus mykiss) contain two calnexin genes which encode distinct proteins." in: Developmental and comparative immunology, Vol. 42, Issue 2, pp. 211-9, (2014) (PubMed).

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: Calnexin

Background: This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described.

Cellular Localization: Endoplasmic reticulum membrane , Melanosome

Molecular Weight: 68 kDa

Gene ID: 821

Pathways: MAPK Signaling, Thyroid Hormone Synthesis