PHGDH antibody (N-Term)

Catalog No. ABIN2855237

The Rabbit Polyclonal anti-PHGDH antibody has been validated for WB, IHC (p), IF, IP, IHC (fro) and ICC. It is suitable to detect PHGDH in samples from Human.

Quick Overview for PHGDH antibody (N-Term) (ABIN2855237)

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHGDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-PHGDH Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to PHGDH (phosphoglycerate dehydrogenase)
PHGDH antibody [N1N2], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human PHGDH. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , 293T , A431 , HeLaS3 , HeLa

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.87 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PHGDH

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: phosphoglycerate dehydrogenase

Background: 3-Phosphoglycerate dehydrogenase (PHGDH, EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Molecular Weight: 57 kDa

Gene ID: 26227

UniProt: O43175

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect