CDC25A antibody (N-Term)
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- Target See all CDC25A Antibodies
- CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CDC25A antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity
- Human, Mouse, Rat
- Characteristics
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Rabbit Polyclonal antibody to CDC25A (cell division cycle 25 homolog A (S. pombe))
CDC25A antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the N-terminus region of human CDC25A. The exact sequence is proprietary.
- Isotype
- IgG
- Top Product
- Discover our top product CDC25A Primary Antibody
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- Application Notes
- WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
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Positive Control: H1299 , MCF-7
Validation: Orthogonal
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
- Alternative Name
- cell division cycle 25A (CDC25A Products)
- Synonyms
- cdc25a2 antibody, cdc25A antibody, sb:cb317 antibody, CDC25A2 antibody, D9Ertd393e antibody, cell division cycle 25A antibody, cell division cycle 25B antibody, cell division cycle 25A S homeolog antibody, CDC25A antibody, cdc25a antibody, cdc25b antibody, Cdc25a antibody, cdc25a.S antibody
- Background
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CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
- Molecular Weight
- 59 kDa
- Gene ID
- 993
- UniProt
- P30304
- Pathways
- Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
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