NLRC4 antibody (N-Term)

Catalog No. ABIN2855371

This Rabbit Polyclonal antibody specifically detects NLRC4 in WB. It exhibits reactivity toward Human.

Quick Overview for NLRC4 antibody (N-Term) (ABIN2855371)

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLRC4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NLRC4 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to CARD12 (NLR family, CARD domain containing 4)
CARD12 antibody [N1], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human CARD12. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MCF-7 , human NLRC4-transfected 293T cells

Validation: Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NLRC4

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Alternative Name: NLR family CARD domain containing 4

Background: In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1 (MIM 602233), NOD1/CARD4 (MIM 605980), and NOD2/CARD15 (MIM 605956). These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.[supplied by OMIM]

Cellular Localization: Cytoplasm

Molecular Weight: 116 kDa

Gene ID: 58484

UniProt: Q9NPP4

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome