POLR2B antibody (N-Term)

Catalog No. ABIN2855420

The Rabbit Polyclonal anti-POLR2B antibody has been validated for WB, ICC, IF and IHC (p). It is suitable to detect POLR2B in samples from Human and Mouse. There is 1 publication available.

Quick Overview for POLR2B antibody (N-Term) (ABIN2855420)

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2B antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-POLR2B Antibody

Binding Specificity: N-Term

Cross-Reactivity: Rat (Rattus), Rhesus Monkey, Cow (Bovine)

Cross-Reactivity (Details): Rat (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to RNA polymerase IIB (DNA-directed RNA polymerase II subunit RPB2)
RPB2 antibody [N1], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: H1299 , BCL-1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.65 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-POLR2B antibody (ABIN2855420)

Jönsson, Ludvik Brattås, Gustafsson, Petri, Yudovich, Pircs, Verschuere, Madsen, Hansson, Larsson, Månsson, Meissner, Jakobsson: "Activation of neuronal genes via LINE-1 elements upon global DNA demethylation in human neural progenitors." in: Nature communications, Vol. 10, Issue 1, pp. 3182, (2019) (PubMed).

Target Details for POLR2B

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Alternative Name: RPB2

Background: This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

Cellular Localization: Nucleus

Molecular Weight: 134 kDa

Gene ID: 5431

Pathways: Regulatory RNA Pathways, DNA Damage Repair