GFR antibody (N-Term)

Catalog No. ABIN2855444

The Rabbit Polyclonal anti-GFR antibody is suitable to detect GFR in samples from Human. It has been validated for WB.

Quick Overview for GFR antibody (N-Term) (ABIN2855444)

Target: GFR (RAPGEF5) (Rap Guanine Nucleotide Exchange Factor (GEF) 5 (RAPGEF5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFR antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GFR Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to RAPGEF5 (Rap guanine nucleotide exchange factor (GEF) 5)
RAPGEF5 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human RAPGEF5. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLaS3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.74 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GFR

Target: GFR (RAPGEF5) (Rap Guanine Nucleotide Exchange Factor (GEF) 5 (RAPGEF5))

Alternative Name: Rap guanine nucleotide exchange factor 5

Background: Members of the RAS (see HRAS, MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM]

Cellular Localization: Nucleus

Molecular Weight: 68 kDa

Gene ID: 9771

UniProt: Q92565

Pathways: RTK Signaling, Neurotrophin Signaling Pathway, Hepatitis C