NCF1 antibody

Catalog No. ABIN2855524

This Rabbit Polyclonal antibody specifically detects NCF1 in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for NCF1 antibody (ABIN2855524)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-NCF1 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to NCF1 (neutrophil cytosolic factor 1)
NCF1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NCF1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Alternative Name: neutrophil cytosolic factor 1

Background: The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.

Cellular Localization: Cytoplasm

Molecular Weight: 45 kDa

Gene ID: 653361

UniProt: P14598

Pathways: PI3K-Akt Signaling