MCM2 antibody (N-Term)
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- Target See all MCM2 Antibodies
- MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MCM2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- Specificity
- IP/MS validation was supported by references (PMID:30377385)
- Cross-Reactivity
- Human
- Characteristics
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Rabbit Polyclonal antibody to MCM2 (minichromosome maintenance complex component 2)
MCM2 antibody [N1N2], N-term - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the N-terminus region of human MCM2. The exact sequence is proprietary.
- Isotype
- IgG
- Top Product
- Discover our top product MCM2 Primary Antibody
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- Application Notes
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
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Positive Control: HCT116
Validation: IP/MS
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))
- Alternative Name
- minichromosome maintenance complex component 2 (MCM2 Products)
- Synonyms
- CG7538 antibody, DmMCM2 antibody, DmMcm2 antibody, DmeMCM2 antibody, Dmel\\CG7538 antibody, MCM2 antibody, MCM2-7 antibody, MCM2_DROME antibody, McM2 antibody, PCR3 antibody, dMCM2 antibody, l(3)rL074 antibody, XMcm2 antibody, bm28 antibody, ccnl1 antibody, cdc19 antibody, cdcl1 antibody, mitotin antibody, cb737 antibody, chunp6905 antibody, BM28 antibody, CCNL1 antibody, CDCL1 antibody, D3S3194 antibody, MITOTIN antibody, MCM7 antibody, AA959861 antibody, AW476101 antibody, Mcmd2 antibody, mKIAA0030 antibody, Minichromosome maintenance 2 antibody, minichromosome maintenance complex component 2 antibody, minichromosome maintenance complex component 2 L homeolog antibody, Mcm2 antibody, mcm2 antibody, MCM2 antibody, mcm2.L antibody
- Background
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The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
- Molecular Weight
- 102 kDa
- Gene ID
- 4171
- UniProt
- P49736
- Pathways
- DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
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