STAT2 antibody (C-Term)

Catalog No. ABIN2855558

The Rabbit Polyclonal anti-STAT2 antibody is suitable to detect STAT2 in samples from Human. It has been validated for WB, IP and IHC (p).

Quick Overview for STAT2 antibody (C-Term) (ABIN2855558)

Target: STAT2 (Signal Transducer and Activator of Transcription 2, 113kDa (STAT2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-STAT2 Antibody

Binding Specificity: C-Term

Specificity: IP/MS validation was supported by references (PMID:30377386)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to STAT2 (signal transducer and activator of transcription 2, 113 kDa)
STAT2 antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human STAT2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat , Raji , K562 , THP-1

Validation: IP/MS, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for STAT2

Target: STAT2 (Signal Transducer and Activator of Transcription 2, 113kDa (STAT2))

Alternative Name: signal transducer and activator of transcription 2

Background: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus.

Cellular Localization: Cytoplasm , Nucleus

Molecular Weight: 98 kDa

Gene ID: 6773

UniProt: P52630

Pathways: JAK-STAT Signaling, Hepatitis C, CXCR4-mediated Signaling Events