ACTN3 antibody (Center)

Catalog No. ABIN2855581

This Rabbit Polyclonal antibody specifically detects ACTN3 in WB and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ACTN3 antibody (Center) (ABIN2855581)

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ACTN3 Antibody

Binding Specificity: Center

Cross-Reactivity: Cow (Bovine)

Cross-Reactivity (Details): Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to alpha Actinin 3 (alpha-actinin-3)
alpha Actinin 3 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Actinin 3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Immunoprecipitation 1:100-1:500* Western blot 1:5000-1:20000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceImmunoprecipitation1:100-1:500* Western blot1:5000-1:20000*

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , PC-12

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ACTN3

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Alternative Name: alpha Actinin 3

Background: Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments

Molecular Weight: 103 kDa

Gene ID: 89

Pathways: Cell-Cell Junction Organization