Nibrin antibody

Catalog No. ABIN2855584

This Rabbit Polyclonal antibody specifically detects Nibrin in WB, IF, ICC and IP. It exhibits reactivity toward Human.

Quick Overview for Nibrin antibody (ABIN2855584)

Target: Nibrin (NBN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nibrin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Grade: KO Validated

Product Details anti-Nibrin Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to NBS1 (nibrin)
NBS1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NBS1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NT2D1 , 293T , A431 , HeLa , HepG2 , 293T

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Nibrin

Target: Nibrin (NBN)

Alternative Name: nibrin

Background: Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.

Cellular Localization: Nucleus (By similarity) , Telomere (By similarity)

Molecular Weight: 85 kDa

Gene ID: 4683

UniProt: O60934

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response