PLAT antibody

Catalog No. ABIN2855635

This Rabbit Polyclonal antibody specifically detects PLAT in WB and IHC (p). It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for PLAT antibody (ABIN2855635)

Target: PLAT (Plasminogen Activator, Tissue (PLAT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLAT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PLAT Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to tPA (plasminogen activator, tissue)
tPA antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human tPA. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , Neuro2A , PC-12 , human plasma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.15 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-PLAT antibody (ABIN2855635)

Lyubenov, Shi, Zhao, Yang, Lei, Mammadova-Bach, de Chiara, Semeraro, Landini, Romagnani, Vörg, Devarapu, Welz, Kiessig, Anders: "Intravenous Glu-plasminogen attenuates cholesterol crystal embolism-induced thrombotic angiopathy, acute kidney injury and kidney infarction." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, (2022) (PubMed).

Target Details for PLAT

Target: PLAT (Plasminogen Activator, Tissue (PLAT))

Alternative Name: plasminogen activator, tissue type

Background: This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Cellular Localization: Secreted , extracellular space

Molecular Weight: 63 kDa

Gene ID: 5327

UniProt: P00750

Pathways: Autophagy, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interactome