PDK3 antibody (Center)

Catalog No. ABIN2855802

The Rabbit Polyclonal anti-PDK3 antibody (ABIN2855802) specifically detects PDK3 in ICC, IHC (p) and IF. The antibody is reactive with Human samples.

Quick Overview for PDK3 antibody (Center) (ABIN2855802)

Target: PDK3 (Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK3 antibody is un-conjugated

Application: Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-PDK3 Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Chicken, Rat (Rattus), Cow (Bovine), Xenopus tropicalis

Cross-Reactivity (Details): Mouse (96 %), Chicken (93 %), Rat (96 %), Bovine (98 %), Xenopus tropicalis (83 %)

Characteristics: Rabbit Polyclonal antibody to PDK3 (pyruvate dehydrogenase kinase, isozyme 3)
PDK3 antibody [N1C2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PDK3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PDK3

Target: PDK3 (Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3))

Alternative Name: PDK3

Background: Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Cellular Localization: Mitochondrion matrix

Molecular Weight: 47 kDa

Gene ID: 5165

Pathways: PI3K-Akt Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect