MMP2 antibody

Catalog No. ABIN2855854

This Rabbit Polyclonal antibody specifically detects MMP2 in WB, IP, IF, ICC, IHC (p) and IHC (fro). It exhibits reactivity toward Human and has been mentioned in 9+ publications.

Quick Overview for MMP2 antibody (ABIN2855854)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MMP2 Antibody

Cross-Reactivity: Human, Mouse, Pig, Rat

Characteristics: Rabbit Polyclonal antibody to MMP2 (matrix metallopeptidase 2 (gelatinase A, 72 kDa gelatinase, 72 kDa type IV collagenase))
MMP2 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MMP2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-MMP2 antibody (ABIN2855854)

Liao, Liang, Li, Wu, Lo, Chang, Hung: "Chrysin Inhibits High Glucose-Induced Migration on Chorioretinal Endothelial Cells via VEGF and VEGFR Down-Regulation." in: International journal of molecular sciences, Vol. 21, Issue 15, (2020) (PubMed).

Nie, Lou, Wang, Cui, Wu, Zhang, Liu, Su, Chang, Guo, Shen, Xing, Li: "Expression, association with clinicopathological features and prognostic potential of CEP55, p-Akt, FoxM1 and MMP-2 in astrocytoma." in: Oncology letters, Vol. 20, Issue 2, pp. 1685-1694, (2020) (PubMed).

Lan, Wang, Lu: "Opsin 3 is a key regulator of ultraviolet A-induced photoageing in human dermal fibroblast cells." in: The British journal of dermatology, Vol. 182, Issue 5, pp. 1228-1244, (2020) (PubMed).

Tsai, Kuo, Lin, Ho, Wang, Chuang, Liou: "Ischemic Stroke Risk Associated with Mitochondrial Haplogroup F in the Asian Population." in: Cells, Vol. 9, Issue 8, (2020) (PubMed).

Xu, Zhang, Chen, Zhang, Zhao, Zhang, Zhao, Deng, Zhou, Wu, Gu: "Nonspecific immunoglobulin G is effective in preventing and treating cancer in mice." in: Cancer management and research, Vol. 11, pp. 2073-2085, (2019) (PubMed).

Liu, Cao, Zhao, Feng, Yu, Li, Teng, Hu, Li: "miR‑338‑3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β‑catenin signaling pathways." in: Molecular medicine reports, Vol. 20, Issue 1, pp. 33-40, (2019) (PubMed).

Lopez-Charcas, Espinosa, Alfaro, Herrera-Carrillo, Ramirez-Cordero, Cortes-Reynosa, Perez Salazar, Berumen, Gomora: "The invasiveness of human cervical cancer associated to the function of NaV1.6 channels is mediated by MMP-2 activity." in: Scientific reports, Vol. 8, Issue 1, pp. 12995, (2019) (PubMed).

Fu, Wang, Cui, Peng, Wang, Geng, Liu, Feng, Song, Li, Zhu, Jiang, Chen: "A novel small-molecule compound targeting CD147 inhibits the motility and invasion of hepatocellular carcinoma cells." in: Oncotarget, Vol. 7, Issue 8, pp. 9429-47, (2017) (PubMed).

Gao, Zhao, Zhang, Lu, Liu, Geng, Huang, Zhang, Lu: "The dual function of PRMT1 in modulating epithelial-mesenchymal transition and cellular senescence in breast cancer cells through regulation of ZEB1." in: Scientific reports, Vol. 6, pp. 19874, (2016) (PubMed).

Target Details for MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Alternative Name: matrix metallopeptidase 2

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Secreted , extracellular space , extracellular matrix

Molecular Weight: 74 kDa

Gene ID: 4313

UniProt: P08253

Pathways: Activation of Innate immune Response