ERCC6 antibody (Internal Region)

Catalog No. ABIN2855858

There are 2+ publications for this product available. The Rabbit Polyclonal anti-ERCC6 antibody is suitable to detect ERCC6 in samples from Human, Rat and Mouse. It has been validated for WB, IHC (p), IP, IF and ICC.

Quick Overview for ERCC6 antibody (Internal Region) (ABIN2855858)

Target: ERCC6 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 6 (ERCC6))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ERCC6 Antibody

Binding Specificity: Internal Region

Characteristics: Rabbit Polyclonal antibody to CSB (excision repair cross-complementing rodent repair deficiency, complementation group 6)
CSB antibody [N2C1], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CSB. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 1XPBS, 0.2M Tris, 0.2M Glycine, 1 mM EGTA, 50 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ERCC6 antibody (ABIN2855858)

Salas-Armenteros, Klunder, Vermeulen, Tresini: "Crosstalk between chromatin state and ATM signalling in DNA damage-induced transcription stress." in: The EMBO journal, Vol. 44, Issue 19, pp. 5564-5594, (2025) (PubMed).

van Sluis, Yu, van der Woude, Gonzalo-Hansen, Dealy, Janssens, Somsen, Ramadhin, Dekkers, Wienecke, Demmers, Raams, Davó-Martínez, Llerena Schiffmacher, van Toorn, Häckes, Thijssen, Zhou, Lammers, Pines, Vermeulen, Pothof, Demmers, van den Berg, Lans, Mar: "Transcription-coupled DNA-protein crosslink repair by CSB and CRL4CSA-mediated degradation." in: Nature cell biology, Vol. 26, Issue 5, pp. 770-783, (2024) (PubMed).

Target Details for ERCC6

Target: ERCC6 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 6 (ERCC6))

Alternative Name: CSB

Background: This gene encodes a DNA-binding protein that is important in transcription-coupled excision repair. The protein has ATP-stimulated ATPase activity, there are contradictory publications reporting presence or absence of helicase activity. The protein appears to interact with several transcription and excision repair proteins, and may promote complex formation at repair sites. Mutations in this gene result in Cockayne syndrome type B.

Cellular Localization: Nucleus

Molecular Weight: 168 kDa

Gene ID: 2074

Pathways: DNA Damage Repair, Chromatin Binding