RFC2 antibody

Catalog No. ABIN2856035

The Rabbit Polyclonal anti-RFC2 antibody has been validated for WB, IF and ICC. It is suitable to detect RFC2 in samples from Human.

Quick Overview for RFC2 antibody (ABIN2856035)

Target: RFC2 (Replication Factor C (Activator 1) 2, 40kDa (RFC2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RFC2 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to RFC2 (replication factor C (activator 1) 2, 40 kDa)
RFC2 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human RFC2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: H1299 , 293T , A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.43 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RFC2

Target: RFC2 (Replication Factor C (Activator 1) 2, 40kDa (RFC2))

Alternative Name: replication factor C subunit 2

Background: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Cellular Localization: Nucleus

Molecular Weight: 39 kDa

Gene ID: 5982

UniProt: P35250

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA