PPP1CC antibody (Center)

Catalog No. ABIN2856137

There are 2+ publications for this product available. The Rabbit Polyclonal anti-PPP1CC antibody is suitable to detect PPP1CC in samples from Human and Mouse. It has been validated for WB, IHC (p), IF and ICC.

Quick Overview for PPP1CC antibody (Center) (ABIN2856137)

Target: PPP1CC (Protein Phosphatase 1, Catalytic Subunit, gamma Isoform (PPP1CC))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1CC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PPP1CC Antibody

Binding Specificity: Center

Cross-Reactivity: Rat (Rattus), Xenopus laevis, Xenopus tropicalis, Chicken, Rhesus Monkey, Cow (Bovine)

Cross-Reactivity (Details): Rat (100 %), Xenopus laevis (100 %), Xenopus Tropicalis (100 %), Chicken (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to PP1C gamma (protein phosphatase 1, catalytic subunit, gamma isozyme)
PP1C gamma antibody [N1C2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PP1C gamma. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-PPP1CC antibody (ABIN2856137)

Kenney, Genheden, Moon, Wang, Foster, Proud: "Eukaryotic elongation factor 2 kinase regulates the synthesis of microtubule-related proteins in neurons." in: Journal of neurochemistry, Vol. 136, Issue 2, pp. 276-84, (2016) (PubMed).

Wei, Ngo, Wu, Lee: "Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint." in: Molecular biology of the cell, Vol. 22, Issue 19, pp. 3584-94, (2012) (PubMed).

Target Details for PPP1CC

Target: PPP1CC (Protein Phosphatase 1, Catalytic Subunit, gamma Isoform (PPP1CC))

Alternative Name: PP1C gamma

Background: Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II.

Molecular Weight: 37 kDa

Gene ID: 5501

Pathways: Cellular Glucan Metabolic Process, Lipid Metabolism