EEF1E1 antibody

Catalog No. ABIN2856150

This anti-EEF1E1 antibody is a Rabbit Polyclonal antibody detecting EEF1E1 in WB, IF, ICC and IHC (p). Suitable for Human.

Quick Overview for EEF1E1 antibody (ABIN2856150)

Target: EEF1E1 (Eukaryotic Translation Elongation Factor 1 epsilon 1 (EEF1E1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF1E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-EEF1E1 Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to EEF1E1 (eukaryotic translation elongation factor 1 epsilon 1)
EEF1E1 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human EEF1E1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , A431 , 293T

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EEF1E1

Target: EEF1E1 (Eukaryotic Translation Elongation Factor 1 epsilon 1 (EEF1E1))

Alternative Name: eukaryotic translation elongation factor 1 epsilon 1

Background: This gene encodes a multifunctional protein that localizes both in the cytoplasm and in the nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage and plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants.

Cellular Localization: Cytoplasm , Nucleus

Molecular Weight: 20 kDa

Gene ID: 9521

UniProt: O43324

Pathways: Positive Regulation of Response to DNA Damage Stimulus