DCLK2 antibody

Catalog No. ABIN2856229

This Rabbit Polyclonal antibody specifically detects DCLK2 in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for DCLK2 antibody (ABIN2856229)

Target: DCLK2 (Doublecortin-Like Kinase 2 (DCLK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCLK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-DCLK2 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to DCAMKL2 (doublecortin-like kinase 2)
DCAMKL2 antibody [N2C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DCAMKL2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A549 , H1299 , HCT116

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.16 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DCLK2

Target: DCLK2 (Doublecortin-Like Kinase 2 (DCLK2))

Alternative Name: doublecortin like kinase 2

Background: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified.

Molecular Weight: 84 kDa

Gene ID: 166614

UniProt: Q8N568