Nodal antibody (Center)

Catalog No. ABIN2856412

This Rabbit Polyclonal antibody specifically detects Nodal in WB, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Nodal antibody (Center) (ABIN2856412)

Target: Nodal (NODAL) (Nodal Homolog (NODAL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nodal antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Nodal Antibody

Binding Specificity: Center

Cross-Reactivity: Cow (Bovine)

Cross-Reactivity (Details): Bovine (93 %)

Characteristics: Rabbit Polyclonal antibody to Nodal (nodal homolog (mouse))
Nodal antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Nodal. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ELISA 1:1000-1:10000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceELISA1:1000-1:10000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: Human ESC

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Nodal

Target: Nodal (NODAL) (Nodal Homolog (NODAL))

Alternative Name: Nodal

Background: The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development.

Cellular Localization: Secreted

Molecular Weight: 40 kDa

Gene ID: 4838

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of Endopeptidase Activity