DENND1B antibody (Center)

Catalog No. ABIN2856495

This anti-DENND1B antibody is a Rabbit Polyclonal antibody detecting DENND1B in WB, ICC and IF. Suitable for Human and Mouse.

Quick Overview for DENND1B antibody (Center) (ABIN2856495)

Target: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DENND1B antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-DENND1B Antibody

Binding Specificity: Center

Characteristics: Rabbit Polyclonal antibody to DENND1B (DENN/MADD domain containing 1B)
DENND1B antibody [N1C1]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DENND1B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.9 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DENND1B

Target: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Alternative Name: DENND1B

Background: Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2, see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM]

Molecular Weight: 48 kDa

Gene ID: 163486