SH3BP1 antibody (C-Term)

Catalog No. ABIN2856521

The Rabbit Polyclonal anti-SH3BP1 antibody is suitable to detect SH3BP1 in samples from Human. It has been validated for WB, IF, ICC and IHC (p).

Quick Overview for SH3BP1 antibody (C-Term) (ABIN2856521)

Target: SH3BP1 (SH3-Domain Binding Protein 1 (SH3BP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH3BP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SH3BP1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to SH3BP1 (SH3-domain binding protein 1)
SH3BP1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SH3BP1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa , Jurkat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SH3BP1

Target: SH3BP1 (SH3-Domain Binding Protein 1 (SH3BP1))

Alternative Name: SH3 domain binding protein 1

Background: Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to the ABL1 proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac.

Molecular Weight: 76 kDa

Gene ID: 23616

UniProt: Q9Y3L3