HS1BP3 antibody (C-Term)

Catalog No. ABIN2856526

This Rabbit Polyclonal antibody specifically detects HS1BP3 in WB, IHC (p), ICC and IF. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for HS1BP3 antibody (C-Term) (ABIN2856526)

Target: HS1BP3 (HCLS1 Binding Protein 3 (HS1BP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HS1BP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: KO Validated

Product Details anti-HS1BP3 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to HS1BP3 (HCLS1 binding protein 3)
HS1BP3 antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HS1BP3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Molt-4

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-HS1BP3 antibody (ABIN2856526)

Holland, Knævelsrud, Søreng, Mathai, Lystad, Pankiv, Bjørndal, Schultz, Lobert, Chan, Zhou, Liest��l, Carlsson, Melia, Di Paolo, Simonsen: "HS1BP3 negatively regulates autophagy by modulation of phosphatidic acid levels." in: Nature communications, Vol. 7, pp. 13889, (2018) (PubMed).

Target Details for HS1BP3

Target: HS1BP3 (HCLS1 Binding Protein 3 (HS1BP3))

Alternative Name: HCLS1 binding protein 3

Background: The protein encoded by this gene shares similarity with mouse Hs1bp3, an Hcls1/Hs1-interacting protein that may be involved in lymphocyte activation.

Molecular Weight: 43 kDa

Gene ID: 64342

UniProt: Q53T59