alpha Tubulin antibody

Catalog No. ABIN2856635

The Rabbit Polyclonal anti-alpha Tubulin antibody has been validated for WB, IF, IHC (p) and ICC. It is suitable to detect alpha Tubulin in samples from Human.

Quick Overview for alpha Tubulin antibody (ABIN2856635)

Target: alpha Tubulin (TUBA1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-alpha Tubulin Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to alpha Tubulin (tubulin, alpha 1b)
alpha Tubulin antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , 293T , A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.81 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: alpha Tubulin

Background: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Gene ID: 10376

Pathways: Microtubule Dynamics