MAPKSP1 antibody

Catalog No. ABIN2856785

The Rabbit Polyclonal anti-MAPKSP1 antibody has been validated for WB and IHC (p). It is suitable to detect MAPKSP1 in samples from Human.

Quick Overview for MAPKSP1 antibody (ABIN2856785)

Target: MAPKSP1 (Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1 (MAPKSP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPKSP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAPKSP1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to MP1 (MAPK scaffold protein 1)
MP1 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Full length human MP1 Recombinant protein.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , Rat2 , Neuro2A , Raw264.7 , C2C12

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.88 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MAPKSP1

Target: MAPKSP1 (Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1 (MAPKSP1))

Alternative Name: late endosomal/lysosomal adaptor, MAPK and MTOR activator 3

Background: This gene encodes a scaffold protein that functions in the extracellular signal-regulated kinase (ERK) cascade. The protein is localized to late endosomes by the mitogen-activated protein-binding protein-interacting protein, and binds specifically to MAP kinase kinase MAP2K1/MEK1, MAP kinase MAPK3/ERK1, and MAP kinase MAPK1/ERK2. Studies of the orthologous gene in mouse indicate that it regulates late endosomal traffic and cell proliferation. Multiple transcript variants are expressed by this gene, but only one variant is thought to express a protein.

Molecular Weight: 14 kDa

Gene ID: 8649

UniProt: Q9UHA4

Pathways: PI3K-Akt Signaling