ATP6V0A4 antibody (N-Term)

Catalog No. ABIN2856797

The Rabbit Polyclonal anti-ATP6V0A4 antibody has been validated for WB. It is suitable to detect ATP6V0A4 in samples from Human.

Quick Overview for ATP6V0A4 antibody (N-Term) (ABIN2856797)

Target: ATP6V0A4 (ATPase, H+ Transporting, Lysosomal V0 Subunit A4 (ATP6V0A4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V0A4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP6V0A4 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Mouse (Murine), Cow (Bovine)

Cross-Reactivity (Details): Mouse (87 %), Bovine (82 %)

Characteristics: Rabbit polyclonal antibody to V-ATPase 116 kDa isoform a4 (ATPase, H+ transporting, lysosomal V0 subunit a4)
V-ATPase 116 kDa isoform a4 antibody [N1N3-2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human V-ATPase 116 kDa isoform a4. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: A431 , HeLa , HepG2 , Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ATP6V0A4

Target: ATP6V0A4 (ATPase, H+ Transporting, Lysosomal V0 Subunit A4 (ATP6V0A4))

Alternative Name: V-ATPase 116 kDa Isoform a4

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing.

Cellular Localization: Apical cell membrane, Multi-pass membrane protein

Molecular Weight: 96 kDa

Gene ID: 50617

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Proton Transport