ATP6V0A4 antibody

Catalog No. ABIN7468025

Product Details anti-ATP6V0A4 Antibody

Target: ATP6V0A4 (ATPase, H+ Transporting, Lysosomal V0 Subunit A4 (ATP6V0A4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V0A4 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ATP6V0A4. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , Molt-4

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.65 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ATP6V0A4

Target: ATP6V0A4 (ATPase, H+ Transporting, Lysosomal V0 Subunit A4 (ATP6V0A4))

Alternative Name: ATPase H+ transporting V0 subunit a4 (ATP6V0A4 Products)

Synonyms: A4 antibody, ATP6N1B antibody, ATP6N2 antibody, RDRTA2 antibody, RTA1C antibody, RTADR antibody, STV1 antibody, VPH1 antibody, VPP2 antibody, Atp6n1b antibody, a4 antibody, ATPase H+ transporting V0 subunit a4 antibody, ATPase, H+ transporting, lysosomal V0 subunit A4 antibody, ATP6V0A4 antibody, Atp6v0a4 antibody

Background:

Synonyms: ATPase H+ transporting V0 subunit a4 , A4 , ATP6N1B , ATP6N2 , RDRTA2 , RTA1C , RTADR , STV1 , VPH1 , VPP2

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq]

Molecular Weight: 96 kDa

Gene ID: 50617

UniProt: Q9HBG4

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Proton Transport