EEF2K antibody (N-Term)

Catalog No. ABIN2856812

The Rabbit Polyclonal anti-EEF2K antibody has been validated for WB, IHC (p), IF and ICC. It is suitable to detect EEF2K in samples from Human.

Quick Overview for EEF2K antibody (N-Term) (ABIN2856812)

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2K antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-EEF2K Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to EEF2K (eukaryotic elongation factor-2 kinase)
eEF-2K antibody [N1N3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human eEF2K. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse liver , A431 , H1299 , HeLa , HepG2 , PC-12

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EEF2K

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Alternative Name: eukaryotic elongation factor 2 kinase

Background: This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.

Molecular Weight: 82 kDa

Gene ID: 29904

UniProt: O00418

Pathways: AMPK Signaling