SSRP1 antibody

Catalog No. ABIN2856824

The Rabbit Polyclonal anti-SSRP1 antibody has been validated for WB, IP and IHC (p). It is suitable to detect SSRP1 in samples from Human.

Quick Overview for SSRP1 antibody (ABIN2856824)

Target: SSRP1 (Structure Specific Recognition Protein 1 (SSRP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SSRP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-SSRP1 Antibody

Specificity: Knockdown/Knockout validation was supported by references (PMID:26990993)

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to SSRP1 (structure specific recognition protein 1)
SSRP1 antibody [N1N3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human SSRP1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Molt-4 , 293T , A431 , HeLa , HepG2 , Jurkat

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SSRP1

Target: SSRP1 (Structure Specific Recognition Protein 1 (SSRP1))

Alternative Name: structure specific recognition protein 1

Background: The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known.

Cellular Localization: Nucleus

Molecular Weight: 81 kDa

Gene ID: 6749

UniProt: Q08945

Pathways: Chromatin Binding