RACGAP1 antibody (N-Term)

Catalog No. ABIN2856889

This Rabbit Polyclonal antibody specifically detects RACGAP1 in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for RACGAP1 antibody (N-Term) (ABIN2856889)

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RACGAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RACGAP1 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to RACGAP1 (Rac GTPase activating protein 1)
RACGAP1 antibody [N1N2], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human RACGAP1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse heart , A431 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-RACGAP1 antibody (ABIN2856889)

Jungas, Perchey, Fawal, Callot, Froment, Burlet-Schiltz, Besson, Davy: "Eph-mediated tyrosine phosphorylation of citron kinase controls abscission." in: The Journal of cell biology, Vol. 214, Issue 5, pp. 555-69, (2017) (PubMed).

Target Details for RACGAP1

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Alternative Name: Rac GTPase activating protein 1

Molecular Weight: 71 kDa

Gene ID: 29127

UniProt: Q9H0H5

Pathways: Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor