PAD4 antibody (Center)

Catalog No. ABIN2856939

Cited in 2+ publications. The Rabbit Polyclonal anti-PAD4 antibody (ABIN2856939) specifically detects PAD4 in WB, ICC, IF, IHC (p) and ChIP. The antibody is reactive with Human and Mouse samples.

Quick Overview for PAD4 antibody (Center) (ABIN2856939)

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

Product Details anti-PAD4 Antibody

Binding Specificity: Center

Characteristics: Rabbit Polyclonal antibody to PAD4 (peptidyl arginine deiminase, type IV)
PAD4 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PAD4.

Application Details

Application Notes: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer ( pH 6.0) or Tris-EDTA buffer ( pH 8.0)], ICC/IF: 1:100-1:1000, ChIP.
Not yet tested in other applications.
Optimal working dilutions should be determined experimentally by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.22 mg/mL

Buffer: PBS

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below.
Avoid multiple freeze-thaw cycles.

References for anti-PAD4 antibody (ABIN2856939)

Indeglia, Leung, Miller, Leu, Dougherty, Clarke, Kirven, Shao, Ke, Lovell, Barnoud, Lu, Lin, Kannan, Battaile, Yang, Batista Oliva, Claiborne, Vogel, Liu, Liu, Nefedova, Cassel, Auslander, Kossenkov, Karanicolas, Murphy: "An African-Specific Variant of TP53 Reveals PADI4 as a Regulator of p53-Mediated Tumor Suppression." in: Cancer discovery, Vol. 13, Issue 7, pp. 1696-1719, (2023) (PubMed).

Li, Lin, Deng, Strnad, Bernabei, Vogl, Burke, Nefedova: "A Novel Peptidylarginine Deiminase 4 (PAD4) Inhibitor BMS-P5 Blocks Formation of Neutrophil Extracellular Traps and Delays Progression of Multiple Myeloma." in: Molecular cancer therapeutics, Vol. 19, Issue 7, pp. 1530-1538, (2020) (PubMed).

Target Details for PAD4

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Alternative Name: PAD4

Gene ID: 23569