CLP1 antibody (Center)

Catalog No. ABIN2856997

This Rabbit Polyclonal antibody specifically detects CLP1 in WB, IHC (p), ICC and IF. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for CLP1 antibody (Center) (ABIN2856997)

Target: CLP1 (Cleavage and Polyadenylation Factor I Subunit 1 (CLP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-CLP1 Antibody

Binding Specificity: Center

Characteristics: Rabbit Polyclonal antibody to CLP1 (CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae))
CLP1 antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CLP1.

Application Details

Application Notes: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer ( pH 6.0) or Tris-EDTA buffer ( pH 8.0)], ICC/IF: 1:100-1:1000.
Not yet tested in other applications.
Optimal working dilutions should be determined experimentally by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.27 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7).
0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below.
Avoid multiple freeze-thaw cycles.

References for anti-CLP1 antibody (ABIN2856997)

Salzman, Nakamura, Nallur, Dookwah, Metheetrairut, Slack, Weidhaas: "miR-34 activity is modulated through 5'-end phosphorylation in response to DNA damage." in: Nature communications, Vol. 7, pp. 10954, (2016) (PubMed).

Target Details for CLP1

Target: CLP1 (Cleavage and Polyadenylation Factor I Subunit 1 (CLP1))

Alternative Name: CLP1

Gene ID: 10978

Pathways: Ribonucleoprotein Complex Subunit Organization