GPER antibody (C-Term)

Catalog No. ABIN2857010

The Rabbit Polyclonal anti-GPER antibody is suitable to detect GPER in samples from Human. It has been validated for WB and IHC.

Quick Overview for GPER antibody (C-Term) (ABIN2857010)

Target: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPER antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: KO Validated

Product Details anti-GPER Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Pig

Characteristics: Rabbit Polyclonal antibody to GPR30 (G protein-coupled estrogen receptor 1)
GPR30 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPR30. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: GFP-human GPR30-transfected 293T cells (No boiling)

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GPER

Target: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Alternative Name: G protein-coupled estrogen receptor 1

Background: This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.

Cellular Localization: Cell membrane, Multi-pass membrane protein , Endoplasmic reticulum membrane, Multi-pass membrane protein (By similarity) , Golgi apparatus membrane, Multi-pass membrane protein (By similarity)

Molecular Weight: 42 kDa

Gene ID: 2852

UniProt: Q99527

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process