GPBAR1 antibody (C-Term)

Catalog No. ABIN2857020

This anti-GPBAR1 antibody is a Rabbit Polyclonal antibody detecting GPBAR1 in WB and IHC (p). Suitable for Human.

Quick Overview for GPBAR1 antibody (C-Term) (ABIN2857020)

Target: GPBAR1 (G Protein-Coupled Bile Acid Receptor 1 (GPBAR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPBAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GPBAR1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to TGR5 (G protein-coupled bile acid receptor 1)
TGR5 antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TGR5. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GPBAR1

Target: GPBAR1 (G Protein-Coupled Bile Acid Receptor 1 (GPBAR1))

Alternative Name: G protein-coupled bile acid receptor 1

Background: This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein.

Cellular Localization: Cell membrane, Multi-pass membrane protein

Molecular Weight: 35 kDa

Gene ID: 151306

UniProt: Q8TDU6

Pathways: WNT Signaling, Hormone Transport, Sensory Perception of Sound