GPBAR1 antibody (AA 30-110)

Catalog No. ABIN7219850

The Rabbit Polyclonal anti-GPBAR1 antibody is suitable to detect GPBAR1 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA and IHC.

Quick Overview for GPBAR1 antibody (AA 30-110) (ABIN7219850)

Target: GPBAR1 (G Protein-Coupled Bile Acid Receptor 1 (GPBAR1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPBAR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-GPBAR1 Antibody

Binding Specificity: AA 30-110

Purpose: Rabbit Anti-TGR5 Polyclonal Antibody

Specificity: TGR5 Polyclonal Antibody detects endogenous levels of TGR5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human TGR5 at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GPBAR1

Target: GPBAR1 (G Protein-Coupled Bile Acid Receptor 1 (GPBAR1))

Alternative Name: TGR5

Background: GPBAR1, TGR5, G-protein coupled bile acid receptor 1, G-protein coupled receptor GPCR19, hGPCR19, Membrane-type receptor for bile acids, M-BAR, hBG37, BG37GPBAR1 encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein.

Gene ID: 151306

UniProt: Q8TDU6

Pathways: WNT Signaling, Hormone Transport, Sensory Perception of Sound