Smooth Muscle Actin antibody (N-Term)

Catalog No. ABIN2857027

This Rabbit Polyclonal antibody specifically detects Smooth Muscle Actin in WB, IF, IHC (p), ICC and IHC (fro). It exhibits reactivity toward Human and has been mentioned in 9+ publications.

Quick Overview for Smooth Muscle Actin antibody (N-Term) (ABIN2857027)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade: KO Validated

Product Details anti-Smooth Muscle Actin Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit polyclonal antibody to alpha Smooth Muscle Actin
alpha Smooth Muscle Actin antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human alpha Smooth Muscle Actin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Rat2 , C8D30 , NIH-3T3 , C2C12

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.37 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Smooth Muscle Actin antibody (ABIN2857027)

Chang, Hsieh, Lu, Chen, Liang, Lin, Hung, Liou, Wu: "Therapeutic Targeting of Aristolochic Acid Induced Uremic Toxin Retention, SMAD 2/3 and JNK/ERK Pathways in Tubulointerstitial Fibrosis: Nephroprotective Role of Propolis in Chronic Kidney Disease." in: Toxins, Vol. 12, Issue 6, (2020) (PubMed).

Wang, Giusti, Ny, de Witte: "Nephrotoxic Effects in Zebrafish after Prolonged Exposure to Aristolochic Acid." in: Toxins, Vol. 12, Issue 4, (2020) (PubMed).

Sun, Lei, Huang et al.: "SOX18 Affects Cell Viability, Migration, Invasiveness, and Apoptosis in Hepatocellular Carcinoma (HCC) Cells by Participating in Epithelial-to-Mesenchymal Transition (EMT) Progression and Adenosine ..." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 25, pp. 6244-6254, (2020) (PubMed).

Stone, Stainier: "Paraxial Mesoderm Is the Major Source of Lymphatic Endothelium." in: Developmental cell, Vol. 50, Issue 2, pp. 247-255.e3, (2020) (PubMed).

Yeh, Chen, Liao, Wu, Huang, Lin: "The Bioactive Core and Corona Synergism of Quantized Gold Enables Slowed Inflammation and Increased Tissue Regeneration in Wound Hypoxia." in: International journal of molecular sciences, Vol. 21, Issue 5, (2020) (PubMed).

Cheong, Lai, Wu: "Connective tissue growth factor mediates transforming growth factor β-induced collagen expression in human endometrial stromal cells." in: PLoS ONE, Vol. 14, Issue 1, pp. e0210765, (2019) (PubMed).

Feng, Cheng, Yang, Zhang, Ji, Zhang, Lin, Li, Xiong, Liu, Jiang: "Guidance to rational use of pharmaceuticals in gallbladder sarcomatoid carcinoma using patient-derived cancer cells and whole exome sequencing." in: Oncotarget, Vol. 8, Issue 3, pp. 5349-5360, (2018) (PubMed).

Marro, Pfefferli, de Preux Charles, Bise, Jaźwińska: "Collagen XII Contributes to Epicardial and Connective Tissues in the Zebrafish Heart during Ontogenesis and Regeneration." in: PLoS ONE, Vol. 11, Issue 10, pp. e0165497, (2017) (PubMed).

McCracken, Catá, Crawford, Sinagoga, Schumacher, Rockich, Tsai, Mayhew, Spence, Zavros, Wells: "Modelling human development and disease in pluripotent stem-cell-derived gastric organoids." in: Nature, Vol. 516, Issue 7531, pp. 400-4, (2014) (PubMed).

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Alternative Name: actin alpha 2, smooth muscle

Background: The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

Cellular Localization: Cytoplasm , cytoskeleton

Molecular Weight: 42 kDa

Gene ID: 59

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development