NHEJ1 antibody

Catalog No. ABIN2857060

Product Details anti-NHEJ1 Antibody

Target: NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NHEJ1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to XLF (nonhomologous end-joining factor 1)
XLF antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human XLF. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NHEJ1

Target: NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))

Alternative Name: non-homologous end joining factor 1 (NHEJ1 Products)

Synonyms: NHEJ1 antibody, zgc:85657 antibody, XLF antibody, 1700029B21Rik antibody, cernunnos antibody, non-homologous end joining factor 1 antibody, non-homologous end joining factor 1 S homeolog antibody, solute carrier family 23 member 3 antibody, nonhomologous end-joining factor 1 antibody, NHEJ1 antibody, nhej1.S antibody, nhej1 antibody, SLC23A3 antibody, Nhej1 antibody

Background: Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.

Cellular Localization: Nucleus

Molecular Weight: 33 kDa

Gene ID: 79840

UniProt: Q9H9Q4

Pathways: DNA Damage Repair