Phosphothreonine-PROLINE antibody

Catalog No. ABIN344517

The Mouse Monoclonal anti-Phosphothreonine-PROLINE antibody (ABIN344517) specifically detects Phosphothreonine-PROLINE in WB, ELISA, IHC and IHC (p).

Quick Overview for Phosphothreonine-PROLINE antibody (ABIN344517)

Target: Phosphothreonine-PROLINE

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Specificity: Detects phospho- threonine only when followed by proline. It reacts with proteins/peptides phosphorylated on the Thr-Pro motif in an otherwise highly context-independent fashion. The phosphospecific antibody does not recognize phospho-threonine in the absence of an adjacent proline. Does not react with phospho-tyrosine, but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e. g., phospho-Elk-1). Recognizes all species.

Purification: Protein A purified

Immunogen: Synthetic phosphothreonine- proline-containing peptides (KLH coupled). Antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody.

Type of Immunogen: Synthetic peptide

Isotype: IgM

Application Details

Application Notes: Approved: ELISA (1:1000), IHC, IHC-P, WB (1:5000)

Usage: Western Blot: 1:5000. Immunohistochemistry (Paraffin): 1:50. ELISA: 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Target Details

Target: Phosphothreonine-PROLINE

Alternative Name: Phospho-Threonine-Proline

Target Type: Chemical