Ig antibody (Light Chain) (HRP)

Catalog No. ABIN288435

The HRP-conjugated Goat Polyclonal anti-Ig antibody (ABIN288435) specifically detects Ig in ELISA, WB, IHC and ICC. The antibody is reactive with Mouse samples.

Quick Overview for Ig antibody (Light Chain) (HRP) (ABIN288435)

Target: Ig

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Ig antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-Ig Antibody (HRP)

Binding Specificity: Light Chain

Specificity: This monospecific antisera recognizes only human ß-2-Microglobulin by IEP analysis.

Cross-Reactivity (Details): This Antibody may cross react with kappa from other species1 ml@ 1 mg/ml

Purification: Affinity chromatography purified

Isotype: IgG

Application Details

Application Notes: ELISA: 1:10,000-1:100,000, ICC: 1:200-1:500, IHC: 1:200-1:500, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS with 0.2 % BSA and 0.1 % Pro-Clean 400.

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Dilute only prior to immediate use.

Storage: 4 °C

Target Details for Ig

Target: Ig

Background: There are two types of light chain in mammals, the kappa chain, encoded by the immunoglobulin kappa locus on chromosome 2, and the lambda chain, encoded by the immunoglobulin lambda locus on chromosome 22. Antibodies are produced by B lymphocytes, each expressing only one class of light chain. Once set, light chain class remains fixed for the life of the B lymphocyte.