IL1A antibody

Catalog No. ABIN289344

The Rabbit Polyclonal anti-IL1A antibody (ABIN289344) specifically detects IL1A in WB, ELISA and InhibAb. The antibody is reactive with Mouse samples.

Quick Overview for IL1A antibody (ABIN289344)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Blocking Antibody (InhibAb)

Product Details anti-IL1A Antibody

Purification: Purified

Immunogen: IL1 a antibody was raised in rabbit using highly pure recombinant murine IL-1 as the immunogen.

Application Details

Application Notes: ELISA: 0.5-2 µg/mL, Inhibition: 0.03-0.05 µg/mL, WB: 0.1-0.2 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water at a concentration of >0.2 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL1a

Background: IL1a is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL1a and IL1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL2 release, B-cell maturation and proliferation, mitogenic FGFlike activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL1beta is a secreted cytokine, IL1a is predominantly a cell-associated cytokine.

Molecular Weight: 17.7 kDa (predicted detection band MW)

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints