SLC6A3 antibody

Catalog No. ABIN289420

Product Details anti-SLC6A3 Antibody

Target: SLC6A3 (Solute Carrier Family 6 (Neurotransmitter Transporter, Dopamine), Member 3 (SLC6A3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: This antibody reacts with Parainfluenza 2 and 3, but does not react with Parainfluenza 1.

Cross-Reactivity: Human, Dog (Canine), Mouse (Murine)

Cross-Reactivity (Details): This DAT peptide sequence is 100 % identical in mouse, 77 % homologous with bovine, monkey, and 72 % in human DAT sequences, respectively.

Purification: Affinity chromatography purified

Immunogen: Dopamine transporter antibody was raised in rabbit using an 18 amino acid peptide of rat DAT conjugated to KLH. as the immunogen.

Isotype: IgG

Application Details

Application Notes: ELISA: 0.5-1 µg/mL, IHC: 2-10 µg/mL, WB: 1-10 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in PBS to a concentration of 1 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 0.1 % BSA.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for SLC6A3

Target: SLC6A3 (Solute Carrier Family 6 (Neurotransmitter Transporter, Dopamine), Member 3 (SLC6A3))

Alternative Name: Dopamine Transporter (SLC6A3 Products)

Background: The dopamine transporter (also known as dopamine active transporter, DAT, SLC6A3) is a membrane-spanning protein that pumps the neurotransmitter dopamine out of the synapse back into cytosol, from which other transporters sequester DA and NE into vesicles for later storage and release. Dopamine reuptake via DAT provides the primary mechanism through which dopamine is cleared from synapses except in the prefrontal cortex, where dopamine uptake via the norepinephrine transporter plays that role.

Pathways: Dopaminergic Neurogenesis