Survivin antibody (AA 125-142)

Catalog No. ABIN289546

This Rabbit Polyclonal antibody specifically detects Survivin in WB. It exhibits reactivity toward Human.

Quick Overview for Survivin antibody (AA 125-142) (ABIN289546)

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Survivin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Survivin Antibody

Binding Specificity: AA 125-142

Specificity: This antibody will recognize wild type Rev protein produced from both in vivo (expression in COS, CV-1, HeLa cell lines) and in vitro (E. coli bacterial expressionsystem and translation products). Very low crossreactivity with other E. coli proteinson western blot. Crossreactivity with other GST fusion proteins will occur unless preabsorbed against GST.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): This antibody reacts with residues 125-142 EETAKKVRRAIEQLAAMD of the human Survivin protein. Cross reacts with mouse and rat Survivin (88% sequenceidentity).

Purification: Affinity chromatography purified

Immunogen: Survivin antibody was raised in rabbit using AA 125-142 EETAKKVRRAIEQLAAMD of the human survivin protein as the immunogen.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Supplied in PBS, with 0.02 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for Survivin

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Alternative Name: Survivin

Background: Survivin is a human gene encoding a structurally unique IAP apoptosis inhibitor. Present during fetal development, survivin is undetectable in terminally differentiated adult tissues. However, survivin becomes prominently expressed in transformed cell lines and in all the most common human cancers of lung, colon, pancreas, prostate and breast, in vivo.

Pathways: Apoptosis, Cell Division Cycle, Nuclear Hormone Receptor Binding