TGFA antibody

Catalog No. ABIN5676024

This Goat Polyclonal antibody specifically detects TGFA in IHC, WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for TGFA antibody (ABIN5676024)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TGFA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Product Details anti-TGFA Antibody

Cross-Reactivity (Details): human TGFalpha 100%. (Neutralizing antibody)We found no crossreactivty to other cytokines such as hTGFß, hEGF, hPDGf, hTNF. hEPO, hIGF, 1mg per ml. hIL-1, hIL-2, hIL-3, hIL-4, hIL-5, hIL-6, hIL-7, hIL-8, hIL-9, hIL-10, hIL-11,and hIL-12

Purification: Purified

Endotoxin Level: <0.1 EU per 1 µg of the antibody as determined by the LAL method.

Immunogen: TGF alpha antibody was raised in goat using E. coli-derived recombinant human TGF-alpha as the immunogen.

Isotype: IgG

Application Details

Application Notes: ELISA: 2-8 µg/mL, IHC: 15 µg/mL, WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with PBS buffer.

Concentration: Lot specific

Buffer: Supplied as a lyophilized powder.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGF alpha

Background: Transforming growth factor alpha (TGF- alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects . TGF alpha stimulates neural cell proliferation in the adult injured brain.

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway