ADAM17 antibody (C-Term)

Catalog No. ABIN290260

This Rabbit Polyclonal antibody specifically detects ADAM17 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for ADAM17 antibody (C-Term) (ABIN290260)

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ADAM17 Antibody

Binding Specificity: C-Term

Specificity: Synthetic peptide corresponding to C-terminal residues of human ADAM 17 (a disintegrin and metalloproteinase domain 17 isoform 1 preproprotein)

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human ADAM17.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1 μg/mL), IHC, IHC-P (20 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.01 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ADAM17

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Alternative Name: ADAM17 / TACE

Background: Name/Gene ID: ADAM17
Subfamily: Metallopeptidase M12B
Family: Protease

Synonyms: ADAM17, CD156B, CD156b antigen, CSVP, ADAM 17, NISBD, TNF-alpha converting enzyme, TNF-alpha convertase, TNF-alpha-converting enzyme, Snake venom-like protease, TACE

Gene ID: 6868

UniProt: P78536

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus