SIRT5 antibody (AA 30-46)

Catalog No. ABIN290374

This Rabbit Polyclonal antibody specifically detects SIRT5 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SIRT5 antibody (AA 30-46) (ABIN290374)

Target: SIRT5 (Sirtuin 5 (SIRT5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIRT5 Antibody

Binding Specificity: AA 30-46

Specificity: A synthetic peptide corresponding to amino acids 30-46 of human SIRT5 was used as immunogen.

Purification: Protein G purified

Immunogen: A synthetic peptide corresponding to amino acids 30-46 of h SIRT5.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for SIRT5

Target: SIRT5 (Sirtuin 5 (SIRT5))

Alternative Name: SIRT5 / Sirtuin 5

Background: Name/Gene ID: SIRT5
Family: ADP-Ribosyltransferase

Synonyms: SIRT5, SIR2L5, Sir2-like 5, SIR2-like protein 5, Sirtuin 5, Sirtuin type 5

Gene ID: 23408

UniProt: Q9NXA8

Pathways: SARS-CoV-2 Protein Interactome